Chapter 12 Biotechnology and its Applications MCQs for Class 12 Biology

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Chapter 12 Biotechnology and its Applications MCQs for Class 12 Biology

Chapter 12 Biotechnology and its Applications MCQs for Class 12 Biology

Here students can practice Chapter 12 Biotechnology and its Applications MCQs for Class 12 Biology. All the MCQs are divided into several sub-pages. Each page has 40 MCQs, for the next page just scroll down and click on the buttons. Also you can practice Class 12 Biology MCQs (Multiple Choice Questions) for other units.

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Q 1.: Chloramphenicol and erythromycin (broad spectrum antibiotics) are produced by :

(A) Streptomyces 

(B) Penicillium 

(C) Rhizobium 

(D) Nitrobacteria 

Option – A 

Q 2.: Ethyl alcohol is commercially obtained from :

(A) Aspergillus 

(B) Penicillium 

(C) Saccharomyces 

(D) Albugo 

Option – C

Q 3.: Which of the following is a recent application of genetic engineering in diagnostic technique ? 

(A) ELISA test 

(B) PCR 


(D) Electrophoresis 

Option – B

Q 4.: Tumor inducing (T i ) plasmid transforms :

(A) Bacteria 

(B) Fungi 

(C) Animals 

(D) Plants 

Option – D

Q 5.: Gene library refers to : 

(A) DNA sequence information maintained in data bank 

(B) DNA fragments maintained in agarose gel 

(C) DNA fragments of a genome maintained by cloning in cultured cell 

(D) Photographs of DNA fragments printed in book 

Option – C

Q 6.: Curdling of milk in small intestine occurs due to action of : 

(A) Rennin 

(B) Trypsin 

(C) Eryptine  

(D) Chymotrypsin 

Option – A 

Q 7.: The Ti plasmid is often used for making transgenic plants. The plasmid is found in  :

(A) Azotobacter 

(B) Rhizobium 

(C) Clostridium 

(D) Agrobacterium 

Option – D

Q 8.: Cultivation of Bt cotton has been much in the news. The prefix Bt means :

(A) ‘Barium treated’ cotton seeds

(B) ‘Bigger thread’ variety of cotton with better tensile strength 

(C) Produced by ‘biotechnology’ using restriction enzymes and ligases 

(D) Carrying an endotoxin gene from Bacillus thuringiensis 

Option – D

Q 9.: The transgenic food crop which may help in solving the problem of night blindness in developing countries is : 

(A) Bt soybean 

(B) Golden rice  

(C) Flavr savr tomatoes 

(D) Starlink maize 

Option – B

Q 10.: Ethyl alcohol is commercially manufactured from :

(A) Sugarcane 

(B) Maize 

(C) Grapes 

(D) Bajra  

Option – A 

Q 11.: In transgenics, expression of transgene in target tissue is determined by : 

(A) Enhancer 

(B) Transgene 

(C) Promoter 

(D) None of these 

Option – C 

Q 12.: Main objective of production/use of herbicide resistant GM crops is to :

(A) Encourage eco-friendly herbicides 

(B) Reduce herbicide accumulation in food articles for heath safety 

(C) Eliminate weeds from the field without the use of manual labour 

(D) Eliminate weeds from the field without the use of herbicides

Option – C 

Q 13.: Bacillus thuringiensis (Bt) strains have been used for designing novel : 

(A) Bio-insecticidal plants 

(B) Bio-mineralization processes 

(C) Bio-fertilizers 

(D) Bio-herbicides 

Option – A 

Q 14.: Which of the following bacteria has found extensive use in genetic engineering work in plants ?

(A) Xanthomonas citri  

(B) Bacillus coagulens 

(C) Clostridium septicum 

(D) Agrobacterium tumefaciens 

Option – D 

Q 15.: Which one of the following is now being commercially produced by biotechnological procedures ? 

(A) Nicotine 

(B) Insulin 

(C) Quinine 

(D) Morphine 

Option – B

Q 16.: Vitamin B12 is produced directly during the course of fermentation by ; 

(A) Rhizopus stolonifer 

(B) Saccharomyces cerevisiae

(C) Penicillium 

(D) Propionibacterium  

Option – D

Q 17.: The antibiotics obtained from Streptomyces are :

(A) Leucomycin , Spiramycin 

(B) Neomycin , Novobiocin 

(C) Erythromycin , Chloramphenicol 

(D) All of the above

Option – D

Q 18.: Transgenic crops developed to tolerate herbicides are :

(A) Tomato and Rice 

(B) Tomato and Tobacco 

(C) Rice and Wheat 

(D) Maize and Sugarcane 

Option – B

Q 19.: What is true about Bt toxin ?

(A) The concerned Bacillus has antitoxins 

(B) Bt protein exist as active toxin in the Bacillus 

(C) The inactivated protoxin gets converted into active form in insect gut. 

(D) The activated toxin enters the ovary of pest to sterilize it and thus prevent its multiplication 

Option – C

Q 20.: Salt tolerant transgenic plant has been developed for : 

(A) Tomato

(B) Brinjal 

(C) Grapes 

(D) Potato 

Option – A

Q 21.: The genetically modified brinjal in India has been developed for : 

(A) Enhancing self-life 

(B) Drought resistance 

(C) Insect resistance 

(D) Enhance mineral content 

Option – D

Q 22.: Which one of the following is now being commercially produced by biotechnological procedures ? 

(A) Insulin 

(B) Quinine 

(C) Morphine 

(D) Nicotine 

Option – A 

Q 23.: Cry I Ab and Cry II Ab produce toxins that control : 

(A) Cotton borer and corn bollworms respectively

(B) Corn borer and cotton bollworms respectively 

(C) Tobacco budworms and nematodes respectively 

(D) Corn borer and tobacco budworms respectively 

Option – B

Q 24.: Probiotics are :

(A) Cancer inducing microbes 

(B) Food allergens 

(C) Safe antibiotics 

(D) Live microbial food supplements 

Option – D

Q 25.: Some of the characteristics of Bt-cotton are : 

(A) High yield and production of toxin protein crystals which kill dipteran pests 

(B) High yield and resistance to boll worm.

(C) Long fiber and resistance to aphids 

(D) Maximum yield, long fiber and resistance to beetle pests 

Option – B

Q 26.: An analytical technique that is used in solving dispute based on DNA polymorphism is called : 

(A) DNA sequencing 

(B) DNA fingerprinting 

(C) DNA amplification 

(D) Both ‘A’ and ‘B’ 

Option – B

Q 27.: Who discovered the superbug ? 

(A) H.G. Khorana 

(B) Dilip Shah 

(C) Anand Mohan Chakarbarty 

(D) Robert willinkan 

Option – C

Q 28.: The two polypeptides of human insulin are linked together by 

(A) Disulphide bonds 

(B) Phosphodiester bonds 

(C) Hydrogen bonds 

(D) Polyamide bonds 

Option – A 

Q 29.: Which part of the tobacco plant is infected by Meloidegyne incognitia

(A) Stem 

(B) Leaf 

(C) Root 

(D) Flower

Option – C 

Q 30.: In Bt cotton, the Bt toxin present in plant tissue as pro-toxin is converted into active toxin due to 

(A) Acidic pH of the insect gut 

(B) Alkaline pH of the insect gut 

(C) Action of gut microorganisms 

(D) Presence of conversion factors in insect gut 

Option – B

Q 31.: Microbes found to be very useful in genetic engineering are : 

(A) Escherichia coli 

(B) Agrobacterium tumefaciens 

(C) Pseudomonas sp.

(D) All of the above 

Option – D 

Q 32.: Exploitation of bio-resources of a nation by multinational companies without authorization from the concerned country is referred to as 

(A) Bioethics 

(B) Bio-patent 

(C) Biopiracy 

(D) Copyright  

Option – C 

Q 33.: What is an antisense technology ? 

(A) When a piece of RNA that is complementary in sequence is used to stop expression of a specific gene 

(B) A cell displaying a foreign antigen used for synthesis of antibodies 

 (C) RNA polymerase producing DNA  

(D) Production of multiple copies of DNA 

Option – A 

Q 34.: Which of the following is commonly used as a vector for introducing a DNA fragment in human lymphocytes ? 

(A) Ti plasmid 

(B) pBr322 

(C) Retrovirus 

(D) λ phage 

Option – C 

Q 35.: In India, the organization responsible for assessing the safety of introducing genetically modified organisms for public use is : 

(A) Genetic Engineering Appraisal Committee (GEAC) 

(B) Research Committee on Genetic Manipulation (RCGM) 

(C) Indian Council of Medical Research (ICMR) 

(D) Council for Scientific and Industrial Research (CSIR) 

Option – A 

Q 36.: Consumption of which one of the following foods can prevent the night blindness associated with vitamin-A deficiency ? 

(A) Bt-Brinjal 

(B) Golden rice 

(C) Flavr savr tomato 

(D) Canolas 

Option – B 

Q 37.: Hepatitis B vaccine is : 

(A) First generation vaccine 

(B) Second generation vaccine 

(C) Third generation vaccine 

(D) mRNA vaccine 

Option – B 

Q 38.: From which one of the following plants, the insecticide pyrethrum is prepared 

(A) Red cabbage 

(B) Crysanthemum 

(C) Argemone 

(D) Butea

Option – B 

Q 39.: RNA interference is used for which of the following purposes in the field of biotechnology ? 

(A) To develop a plant tolerant to abiotic stresses 

(B) To reduce post-harvest losses 

(C) To enhance the mineral usage by the plant 

(D) To develop a pest-resistance plant against infestation by nematode 

Option – D 

Q 40.: For effective treatment of the disease, early diagnosis and understanding its pathophysiology is very important. Which of the following molecular diagnostic techniques is very useful of early detection ? 

(A) Enzyme-linked Immunosorbent Assay 

(B) Southern Blotting 

(C) Western Blotting 

(D) Northern Blotting 

Option – A 

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We hope that these Chapter 12 Biotechnology and its Applications MCQs for Class 12 Biology helped you in learning new concepts. All questions are based on NCERT syllabus and some of them have been asked in various competitive exams. You can go to the next pages to practice more for Chapter 12 Biotechnology and its Applications MCQs for Class 12 Biology, click on the question range given above.

Also you can practice Class 12 Biology MCQs (Multiple Choice Questions) for other chapters.