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Chapter 12 Biotechnology and its Applications MCQs for Class 12 Biology
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Q 1.: Chloramphenicol and erythromycin (broad spectrum antibiotics) are produced by :
Q 2.: Ethyl alcohol is commercially obtained from :
Q 3.: Which of the following is a recent application of genetic engineering in diagnostic technique ?
(A) ELISA test
Q 4.: Tumor inducing (T i ) plasmid transforms :
Q 5.: Gene library refers to :
(A) DNA sequence information maintained in data bank
(B) DNA fragments maintained in agarose gel
(C) DNA fragments of a genome maintained by cloning in cultured cell
(D) Photographs of DNA fragments printed in book
Q 6.: Curdling of milk in small intestine occurs due to action of :
Q 7.: The Ti plasmid is often used for making transgenic plants. The plasmid is found in :
Q 8.: Cultivation of Bt cotton has been much in the news. The prefix Bt means :
(A) ‘Barium treated’ cotton seeds
(B) ‘Bigger thread’ variety of cotton with better tensile strength
(C) Produced by ‘biotechnology’ using restriction enzymes and ligases
(D) Carrying an endotoxin gene from Bacillus thuringiensis
Q 9.: The transgenic food crop which may help in solving the problem of night blindness in developing countries is :
(A) Bt soybean
(B) Golden rice
(C) Flavr savr tomatoes
(D) Starlink maize
Q 10.: Ethyl alcohol is commercially manufactured from :
Q 11.: In transgenics, expression of transgene in target tissue is determined by :
(D) None of these
Q 12.: Main objective of production/use of herbicide resistant GM crops is to :
(A) Encourage eco-friendly herbicides
(B) Reduce herbicide accumulation in food articles for heath safety
(C) Eliminate weeds from the field without the use of manual labour
(D) Eliminate weeds from the field without the use of herbicides
Q 13.: Bacillus thuringiensis (Bt) strains have been used for designing novel :
(A) Bio-insecticidal plants
(B) Bio-mineralization processes
Q 14.: Which of the following bacteria has found extensive use in genetic engineering work in plants ?
(A) Xanthomonas citri
(B) Bacillus coagulens
(C) Clostridium septicum
(D) Agrobacterium tumefaciens
Q 15.: Which one of the following is now being commercially produced by biotechnological procedures ?
Q 16.: Vitamin B12 is produced directly during the course of fermentation by ;
(A) Rhizopus stolonifer
(B) Saccharomyces cerevisiae
Q 17.: The antibiotics obtained from Streptomyces are :
(A) Leucomycin , Spiramycin
(B) Neomycin , Novobiocin
(C) Erythromycin , Chloramphenicol
(D) All of the above
Q 18.: Transgenic crops developed to tolerate herbicides are :
(A) Tomato and Rice
(B) Tomato and Tobacco
(C) Rice and Wheat
(D) Maize and Sugarcane
Q 19.: What is true about Bt toxin ?
(A) The concerned Bacillus has antitoxins
(B) Bt protein exist as active toxin in the Bacillus
(C) The inactivated protoxin gets converted into active form in insect gut.
(D) The activated toxin enters the ovary of pest to sterilize it and thus prevent its multiplication
Q 20.: Salt tolerant transgenic plant has been developed for :
Q 21.: The genetically modified brinjal in India has been developed for :
(A) Enhancing self-life
(B) Drought resistance
(C) Insect resistance
(D) Enhance mineral content
Q 22.: Which one of the following is now being commercially produced by biotechnological procedures ?
Q 23.: Cry I Ab and Cry II Ab produce toxins that control :
(A) Cotton borer and corn bollworms respectively
(B) Corn borer and cotton bollworms respectively
(C) Tobacco budworms and nematodes respectively
(D) Corn borer and tobacco budworms respectively
Q 24.: Probiotics are :
(A) Cancer inducing microbes
(B) Food allergens
(C) Safe antibiotics
(D) Live microbial food supplements
Q 25.: Some of the characteristics of Bt-cotton are :
(A) High yield and production of toxin protein crystals which kill dipteran pests
(B) High yield and resistance to boll worm.
(C) Long fiber and resistance to aphids
(D) Maximum yield, long fiber and resistance to beetle pests
Q 26.: An analytical technique that is used in solving dispute based on DNA polymorphism is called :
(A) DNA sequencing
(B) DNA fingerprinting
(C) DNA amplification
(D) Both ‘A’ and ‘B’
Q 27.: Who discovered the superbug ?
(A) H.G. Khorana
(B) Dilip Shah
(C) Anand Mohan Chakarbarty
(D) Robert willinkan
Q 28.: The two polypeptides of human insulin are linked together by
(A) Disulphide bonds
(B) Phosphodiester bonds
(C) Hydrogen bonds
(D) Polyamide bonds
Q 29.: Which part of the tobacco plant is infected by Meloidegyne incognitia ?
Q 30.: In Bt cotton, the Bt toxin present in plant tissue as pro-toxin is converted into active toxin due to
(A) Acidic pH of the insect gut
(B) Alkaline pH of the insect gut
(C) Action of gut microorganisms
(D) Presence of conversion factors in insect gut
Q 31.: Microbes found to be very useful in genetic engineering are :
(A) Escherichia coli
(B) Agrobacterium tumefaciens
(C) Pseudomonas sp.
(D) All of the above
Q 32.: Exploitation of bio-resources of a nation by multinational companies without authorization from the concerned country is referred to as
Q 33.: What is an antisense technology ?
(A) When a piece of RNA that is complementary in sequence is used to stop expression of a specific gene
(B) A cell displaying a foreign antigen used for synthesis of antibodies
(C) RNA polymerase producing DNA
(D) Production of multiple copies of DNA
Q 34.: Which of the following is commonly used as a vector for introducing a DNA fragment in human lymphocytes ?
(A) Ti plasmid
(D) λ phage
Q 35.: In India, the organization responsible for assessing the safety of introducing genetically modified organisms for public use is :
(A) Genetic Engineering Appraisal Committee (GEAC)
(B) Research Committee on Genetic Manipulation (RCGM)
(C) Indian Council of Medical Research (ICMR)
(D) Council for Scientific and Industrial Research (CSIR)
Q 36.: Consumption of which one of the following foods can prevent the night blindness associated with vitamin-A deficiency ?
(B) Golden rice
(C) Flavr savr tomato
Q 37.: Hepatitis B vaccine is :
(A) First generation vaccine
(B) Second generation vaccine
(C) Third generation vaccine
(D) mRNA vaccine
Q 38.: From which one of the following plants, the insecticide pyrethrum is prepared
(A) Red cabbage
Q 39.: RNA interference is used for which of the following purposes in the field of biotechnology ?
(A) To develop a plant tolerant to abiotic stresses
(B) To reduce post-harvest losses
(C) To enhance the mineral usage by the plant
(D) To develop a pest-resistance plant against infestation by nematode
Q 40.: For effective treatment of the disease, early diagnosis and understanding its pathophysiology is very important. Which of the following molecular diagnostic techniques is very useful of early detection ?
(A) Enzyme-linked Immunosorbent Assay
(B) Southern Blotting
(C) Western Blotting
(D) Northern Blotting
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